Defining with images. (A) horizontal (B) frontal and (C) sagital sections through a standard reference Drosophila brain in which the lateral horn region is highlighted in purple with boundaries defined by the BrainName standardc. We record a formal connection between an OWL individual representing the painted region and the class as: ‘lateral horn’ EquivalentTo has_reference_image value ‘BrainName exemplar lateral horn’. (D) shows an image of single neuron that has been registered to the standard brain. Image analysis has determined overlap between the neuron and the region defined as lateral horn in the standard. Based on this, the neuron in the image has been annotated with the axiom: SubClassOf overlaps some ‘lateral horn’. (Painting of standard brain by A.Jennet and K.Shinomiya. The single neuron image in panel C was derived from a neuron imaged by FlyCircuit  with registration and image analysis by MC and Gregory SXE Jefferis).