Table 2 Metadata represented in SP-Document

Bibliographic metadata

sp:title of the protocol

Extraction of total RNA from fresh/frozen tissue (FT)

sp:author name

“Kim M. Linton”, “Yvonne Hey”, “Sian Dibben”, “Crispin J. Miller”, “Anthony J. Freemont”, “John A. Radford”,

and “Stuart D. Pepper”

sp:protocol identifier

DOI:10.2144/000113260

Descriptive metadata

sp:application of the protocol

“Methods comparison for high-resolution transcriptional analysis of archival material on Affymetrix Plus 2.0 and Exon

1.0 microarrays”

sp:provenance of the protocol

“The extraction method (steps 2–21) is taken from the method supplied with TRIzol reagent Invitrogen, Paisley, UK).”

Metadata about the materials

sp:specimen name

“tumor tissue”

sp:reagent name

“TRIzol”, “Chloroform”, “Ethyl alcohol”, “Isopropyl alcohol”

sp:manufacturer name

“Invitrogen”, “Sigma-Aldrich”

sp:equipment or supplies name

“Tissue storage container”, “Homogenizer blades”, “Forceps”, “Scalpel”, “Scalpel holder”