From: Using semantics for representing experimental protocols
Sample | Whole organism | Scientific name: Arabidopsis |
thaliana, Oriza sativa, mangifera | ||
indica, Mus musculus. | ||
Common names: Mousear Cress, | ||
rice, mango, mouse. | ||
Anatomical part | Leaf, stem, cells, tissues, | |
membranes, organs, skeletal | ||
system, muscular system, | ||
nervous system, reproductive | ||
system, cardiovascular system, etc. | ||
Biomolecules | Nucleic acids: Deoxyribonucleic acid | |
(DNA) and ribonucleic acid (RNA). | ||
Proteins: enzymes, structural or | ||
support proteins (keratin, elastin, | ||
collagen), antibodies, hormones, etc. | ||
Body fluids | Blood serum, saliva, semen, amniotic | |
fluid, cerebrospinal fluid, gastric | ||
acid, etc. | ||
Instrument | High-throughput | Liquid Handling Platforms, Real-Time |
equipment | PCR Detection System, Microplate | |
Reader, etc. | ||
Instruments | Goggles, Bunsen burner, spot plate, | |
pipet, forceps, test tube rack, mortar | ||
and pestle, etc. | ||
Laboratory glassware | Beaker, Erlenmeyer flask, graduated | |
cylinder, volumetric flask, etc. | ||
Standard equipment | Balances, shakers, centrifuges, | |
refrigerators, incubators, | ||
thermocyclers, fume hood, etc. | ||
Consumables | Weighing dishes, pipette tips, gloves, | |
syringes, petri dishes, test tubes, | ||
micro centrifuge tubes, glass slides, | ||
filter paper, etc. | ||
Reagents | Chemical | Glucose, ethanol, glycerol, chloroform, |
compound/Substance | acetic acid, isopropyl alcohol, etc. | |
Solutions/buffers | 70% ethanol, 10X PCR buffer, | |
phenol:chloroform:isoamyl | ||
alcohol, etc. | ||
Cell culture media | Nutrient media, minimal media, | |
selective media, differential media, etc. | ||
Objective | Part of discourse | Here we present a detailed protocol |
for Smart-seq2 that allows the | ||
generation of full-length cDNA and | ||
sequencing libraries by using | ||
standard reagents |