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Fig. 10 | Journal of Biomedical Semantics

Fig. 10

From: Comprehensive anatomic ontologies for lung development: A comparison of alveolar formation and maturation within mouse and human lung

Fig. 10

Immunofluorescence analysis of SFTPC and HOPX expression in the E16.5 mouse lung. Triple-labeled section of an E16.5, C57BL/6, fetal mouse lung stained for HOPX (red), SFTPC (green), ACTA2 (white), and counterstained with DAPI (blue) to visualize the nuclei. HOPX (intense bright red/magenta cytoplasmic staining) is expressed in epithelial cells of the proximal acinar tubules (yellow arrows), while SFTPC (multiple bright green punctate staining) is expressed in epithelial cells of the distal acinar tubules/buds (green arrows). Between the proximal and distal regions of the acinar tubules, there is a transition zone (white arrowheads) with less intense HOPX staining and fewer SFTPC puncta in the cytoplasm of the epithelial cells. Original magnification = 60X. Original image provided by J. A. Kitzmiller and J. A. Whitsett and can be found at the CCHMC LGEA web portal, https://research.cchmc.org/lungimage/ [92, 93]

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