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Fig. 5 | Journal of Biomedical Semantics

Fig. 5

From: Anatomy and the type concept in biology show that ontologies must be adapted to the diagnostic needs of research

Fig. 5

A-FAllium cepa (Amaryllidacea, Magnoliopsida), nucleus of epidermis cell visualized using bright-field (A, B), Nomarski (C), phase contrast microscopy (D), phase contrast plus fluorescence microscopy (E), and fluorescence microscopy (F). Nucleus was stained blue with Hoechst 33342 in E and F. Note prominent nucleoli in B and C. G-LRiseriellus occultus (Lineidae, Nemertea), fertilized egg cells. Due to the yolk content, the nucleus cannot be seen, neither with Nomarski contrast (G) nor with phase contrast microscopy, without being specifically stained (I). Hoechst 33342 stained chromosomes (blue, arrows) can be seen under a fluorescence microscope (H, L) that was combined with phase contrast microscopy in K. Note the fully condensed chromatin in the polar body (small arrow in H). M-RAllium cepa, root meristem, Nomarski microscopy, nuclei stained in red with Schneider carmine dye (carmine acetic acid). M differentiated nucleus. N-R mitotic stages (asterisks), a nuclear membrane is absent. N metaphase, O anaphase, P metaphase, Q late anaphase, R telophase. S-U Histological sections, Azan staining. SClymenura clypeata (Maldanidae, Annelida). Coelom with sperm cells (arrows) and adjacent gut epithelium (asterisks mark nuclei). TSalamander salamander (Amphibia, Craniota). Gut epithelium (asterisks mark nuclei, note red nucleolus). UBufo bufo (Amphibia, Craniota), tadpole. Medullary cord, ganglion cells (asterisks mark nuclei, note red nucleolus). V-Y Transmission electron microscopy, ultrathin (70 nm) sections, uranyl acetate plus lead citrate staining. VAchelia brevicauda (Pantopoda, Arthropoda), Embryo. Euchromatic (a) and heterochromatic (b) nuclei (asterisk marks nucleolus). WEpiperipatus biolleyi (Onychophora, Euarthropoda), embryo, epidermis with mitotic (a) and large heterochromatic (b) nuclei. XTetrastemma cerasinum (Nemertea). Meiotic cell (asterisk). YAmphiporus lactifloreus (Nemertea). Sperm nuclei (black)

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