Screenshots of AISO demonstrating the segmentation process. (A) Open an image file in AISO (e.g. a Chamerion angustifolium flower) (B) Mark the “foreground” (red line): the aspect of the image you would like to segment. (C) Mark the “background” (blue line): the area of the image you want to ignore. (NOTE: Auto-segmentation occurs after the background is marked; further refinement of both foreground and background is possible.) (D) Label the new image segment with an ontology term selected from the integrated web service query interface. (E) Screenshot of the annotation panel of AISO showing a selected ontology term, its definition and synonyms, and designated species (provided via web services), as well as curator, collection, and comment information entered by the user.